Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine
- Authors
- Yang, Zheng-You; Shim, Won-Bo; Kim, Min-Gon; Lee, Kyu-Ho; Kim, Keun-Sung; Kim, Kwang-Yup; Kim, Cheol-Ho; Ha, Sang-Do; Chung, Duck-Hwa
- Issue Date
- Apr-2007
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- ELISA; sulfamethazine; antimicrobial; monoclonal antibody; scFv antibody
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.4, pp 571 - 578
- Pages
- 8
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 17
- Number
- 4
- Start Page
- 571
- End Page
- 578
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24107
- ISSN
- 1017-7825
1738-8872
- Abstract
- A monoclonal antibody (mab) against the antimicrobial sulfamethazine was prepared and characterized by an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA). Sulfamethazine in the range of 0.2 and 45 ng/ml could be determined with the mab by IC-ELISA. cDNAs encoding a variable heavy chain and variable light chain of the mab were cloned to produce recombinant antibodies using phage display technology. Following phage rescue and three rounds of panning, a single-chain variable fragment (scFv) antibody with high sulfamethazine-binding affinity was obtained. ELISA analysis revealed that scFv antibody and parent mab showed similar, but not identical, characteristics. The IC,, value by IC-ELISA with scFv antibody was 4.8 ng/ml, compared with 1.6 ng/ml with the parent mab. Performances of the assays in the presence of milk matrix were compared; the mab-based assay was less affected than the scFv-based assay. Sixty milk samples were analyzed by mab-based IC-ELISA, and four samples were sulfamethazine positive; these results were favorably correlated with those obtained by HPLC.
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Collections - Graduate School > ETC > 1. Journal Articles
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