Identification of duck liver-expressed antimicrobial peptide 2 and characterization of its bactericidal activityopen access
- Authors
- Hong, Yeojin; Truong, Anh Duc; Lee, Janggeun; Lee, Kyungbaek; Kim, Geun-Bae; Heo, Kang-Nyeong; Lillehoj, Hyun S.; Hong, Yeong Ho
- Issue Date
- Jul-2019
- Publisher
- ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC
- Keywords
- Antimicrobial Peptides; Liver-expressed Antimicrobial Peptide 2 (LEAP-2); Duck; Disulfide Bond; Pathogens
- Citation
- ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, v.32, no.7, pp 1052 - 1061
- Pages
- 10
- Journal Title
- ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES
- Volume
- 32
- Number
- 7
- Start Page
- 1052
- End Page
- 1061
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/32697
- DOI
- 10.5713/ajas.18.0571
- ISSN
- 1011-2367
1976-5517
- Abstract
- Objective: This study was conducted to identify duck liver-expressed antimicrobial peptide 2 (LEAP-2) and demonstrate its antimicrobial activity against various pathogens. Methods: Tissue samples were collected from 6 to 8-week-old Pekin ducks (Anas platyrhynchos domesticus), total RNA was extracted, and cDNA was synthesized. To confirm the duck LEAP-2 transcript expression levels, quantitative real-time polymerase chain reaction was conducted. Two kinds of peptides (a linear peptide and a disulfide-type peptide) were synthesized to compare the antimicrobial activity. Then, antimicrobial activity assay and fluorescence microscopic analysis were conducted to demonstrate duck LEAP-2 bactericidal activity. Results: The duck LEAP-2 peptide sequence showed high identity with those of other avian species (>85%), as well as more than 55% of identity with mammalian sequences. LEAP-2 mRNA was highly expressed in the liver with duodenum next, and then followed by lung, spleen, bursa and jejunum and was the lowest in the muscle. Both of LEAP-2 peptides efficiently killed bacteria, although the disulfide-type LEAP-2 showed more powerful bactericidal activity. Also, gram-positive bacteria was more susceptible to duck LEAP-2 than gram-negative bacteria. Using microscopy, we confirmed that LEAP-2 peptides could kill bacteria by disrupting the bacterial cell envelope. Conclusion: Duck LEAP-2 showed its antimicrobial activity against both gram-positive and gram-negative bacteria. Disulfide bonds were important for the powerful killing effect by disrupting the bacterial cell envelope. Therefore, duck LEAP-2 can be used for effective antibiotics alternatives.
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