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벼 렉틴 유전자의 클로닝 및 대장균에서의 발현Molecular Cloning and Expression of Rice Lectin in Escherichia coli
- Issue Date
- Aug-2002
- Publisher
- 대한약학회
- Keywords
- Lectin; rice; gene cloning; expression in E.coli
- Citation
- 약 학 회 지, v.46, no.4, pp 270 - 275
- Pages
- 6
- Journal Title
- 약 학 회 지
- Volume
- 46
- Number
- 4
- Start Page
- 270
- End Page
- 275
- ISSN
- 0377-9556
2383-9457
- Abstract
- The lectin gene from rice was amplified by the polymerase chain reaction. The amplified DNA was inserted into the expression vector pET26b and expressed it as a fusion protein with polyhistidine sequences in Escherichia coli. The recombinant protein was produced by induction with 0.4 mM isopropyl- β -D-thiogalactopyranoside at 37 ℃ and purified by an immobilized metal affinity chromatography. The recombinant protein was found to have lectin activity by the hemagglutination inhibition assay. The hemagglutination activity of the recombinant protein was optimal at pH 4.0-7.0 and was dependent on Ca2+ and Mn 2+.
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