Pseudorivibacter rhizosphaerae gen. Nov., sp. nov., isolated from rhizosphere soil of camellia sinensis (l.) o. ktze and emended description of the genus rivibacter
- Authors
- Zhou, Xing-Kui; Li, Qing-Qing; Dong, Li-Min; Yang, Deng-Qiang; Liu, Lan; Fang, Bao-Zhu; Jeon, Che Ok; Li, Wen-Jun; Duan, Yan-Qing
- Issue Date
- 2020
- Publisher
- Microbiology Society
- Keywords
- Camellia sinensis (L.) O; Ktze; Nov; Phylogenomic tree; Polyhasic taxonomy; Pseudorivibacter rhizosphaerae sp
- Citation
- International Journal of Systematic and Evolutionary Microbiology, v.70, no.2, pp 1071 - 1078
- Pages
- 8
- Journal Title
- International Journal of Systematic and Evolutionary Microbiology
- Volume
- 70
- Number
- 2
- Start Page
- 1071
- End Page
- 1078
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/38340
- DOI
- 10.1099/ijsem.0.003878
- ISSN
- 1466-5026
1466-5034
- Abstract
- A Gram-stain-negative, facultative anaerobic, motile and straight rod-shaped bacterium, designated strain C1-9T, was isolated from rhizosphere soil of Camellia sinensis (L.) O. Ktze collected from a tea garden in Huize, south-western PR China. Cells were oxidase-positive and catalase-negative. Growth occurred at 20–40 °C and pH 6.0–10.0, with an optimal growth at 30 °C and pH 7.0. The respiratory quinone was detected as ubiquinone-8 (Q-8). The major fatty acids were identified as summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0 and summed feature 8 (C18:1 ω7c or C18:1 ω6c). The cellular polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified phospholipids, two unidentified lipids, one unidentified aminophospholipid and one unidentified aminolipid. The polyamine types were detected as 1,8-diaminooctane and 2-hydroxyputrescine. The genomic DNA G+C content was 68.6 mol%. Based on the results of 16S rRNA gene sequence analysis, strain C1-9T (MF687442) showed highest sequence similarity to Rivibacter subsaxonicus DSM 19570T (97.1%). The phylogenetic tree based on 16S rRNA gene sequences showed that strain C1-9T clustered close to R. subsaxonicus DSM 19570T, Methylibium petroleiphilum CCTCC AB 2014193T and species belonging to the genera Rhizobacter and Piscinibacter. The phylogenomic tree indicated that strain C1-9T formed a clade with R. subsaxonicus. The average nucleotide identity value was 76.0% between strain C1-9T and R. subsaxonicus DSM 19570T, which is lower than the prokaryotic species delineation threshold of 95.0–96.0%. The polyphasic taxonomic characteristics indicated that strain C1-9T represents a novel species of a new genus within the order Burkholderiales, for which the name Pseudorivibacter rhizosphaerae gen. nov., sp. nov. (type strain C1-9T = KCTC 62325T=CGMCC 1.13864T) is proposed. © 2020 Sun Yat-Sen University, PR China.
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