Combination of paper membrane-based filtration and ultrafiltration to enhance the detection of MNV, HAV, and HCoV from soil-rich post-washing wateropen access
- Authors
- Wang, Z.; Jung, S.; Yeo, D.; Woo, S.; Seo, Y.; Hossain, M.I.; Kwon, H.; Jeong, M.-I.; Choi, C.
- Issue Date
- Dec-2022
- Publisher
- Elsevier Inc.
- Keywords
- Membrane system; Virology
- Citation
- iScience, v.25, no.12
- Journal Title
- iScience
- Volume
- 25
- Number
- 12
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68526
- DOI
- 10.1016/j.isci.2022.105640
- ISSN
- 2589-0042
2589-0042
- Abstract
- Risk-assessing and controlling virus transmission from soil-rich post-washing water (PWW) are crucial during harvesting raw vegetables. However, viruses are normally difficult to concentrate because of their low concentrations and complex backgrounds. Here, ultrafiltration (UF), virus adsorption-elution (VIRADEL), and optimized paper filtration-coupled ultrafiltration (PFC-UF) methods were employed to evaluate the recovery of non-enveloped murine norovirus (MNV-1), hepatitis A virus (HAV), and enveloped human coronavirus 229E (HCoV-229E) from soil-rich PWW. Among the three methods, PFC-UF outperformed the other methods in the recovery of viruses from PWW with soil content. Under the highest soil condition with virus seeded at a titer of 102 plaque-forming unit (PFU) or TCID50, the PFC-UF method exhibited an exceedingly consistent recovery rate of 78.8 ± 13.3 (MNV-1) and 44.4 ± 25.2% (HAV). However, the recovery of enveloped HCoV-229E was inferior to non-enveloped viruses. Overall, PFC-UF provided a reliable method for recovering viruses in soil-rich PWW. © 2022 The Author(s)
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