Expression of interferon-gamma and tumour necrosis factor-alpha in pigs experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV)
- Authors
- Choi, C; Cho, WS; Kim, B; Chae, C
- Issue Date
- Aug-2002
- Publisher
- ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
- Citation
- JOURNAL OF COMPARATIVE PATHOLOGY, v.127, no.2-3, pp 106 - 113
- Pages
- 8
- Journal Title
- JOURNAL OF COMPARATIVE PATHOLOGY
- Volume
- 127
- Number
- 2-3
- Start Page
- 106
- End Page
- 113
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/68758
- DOI
- 10.1053/jcpa.2002.0566
- ISSN
- 0021-9975
1532-3129
- Abstract
- The expression of mRNA encoding interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) was studied, by reverse transcription-polymerase chain reaction and by in-situ hybridization with a non-radioactive digoxigenin-labelled probe, in formalin-fixed, paraffin wax-embedded lung tissue from pigs experimentally infected with a Korean isolate (North American genotype) of PRRSV. Both IFN-gamma and TNF-alpha mRNA were detected in the lung as early as 1 day post-inoculation (dpi) and up to 10 dpi. Hybridization signals for IFN-gamma and TNF-alpha were usually associated with inflammation, in particular with macrophages within alveolar septa. Expression of these cytokines was minimal in non-lesional lung of PRRSV-infected pigs and in normal lung from control pigs. The results suggest that IFN-gamma and TNF-alpha play a role in pulmonary defence against PRRSV. (C) 2002 Elsevier Science Ltd. All rights reserved.
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