Synergistic attenuation of ovariectomy-induced bone loss by combined use of fish oil and 17β-oestradiol
- Authors
- Jin, Youri; Lee, Myoungsook; Park, Yongsoon
- Issue Date
- Feb-2017
- Publisher
- CAMBRIDGE UNIV PRESS
- Keywords
- 17β-Oestradiol-3-benzoate; n-3 PUFA; Bone loss; Runt-related transcription factor 2; IL-1β
- Citation
- BRITISH JOURNAL OF NUTRITION, v.117, no.4, pp.479 - 489
- Indexed
- SCIE
SCOPUS
- Journal Title
- BRITISH JOURNAL OF NUTRITION
- Volume
- 117
- Number
- 4
- Start Page
- 479
- End Page
- 489
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/152966
- DOI
- 10.1017/S0007114517000344
- ISSN
- 0007-1145
- Abstract
- Oestrogen and n-3 PUFA, especially EPA and DHA, have been reported to have beneficial effects on bone loss. Thus, the purpose of the present study was to investigate the synergistic bone-protective mechanism of combined treatments of EPA+DHA supplementation and oestrogen injection in ovariectomised rats. Rats were fed a modified American Institute of Nutrition-93G diet with 0 %, 1 % or 2 % n-3 PUFA (EPA+DHA) relative to the total energy intake for 12 weeks. Rats were surgically ovariectomised at week 8, and after a 1-week recovery period rats were injected with either 17β-oestradiol-3-benzoate (E₂) or maize oil for the last 3 weeks. Combined use of n-3 PUFA and E₂ synergistically increased femoral cortical bone volume, bone mineral content and the bone expression of runt-related transcription factor 2 (RUNX2), but decreased the bone expression of IL-1β. Both n-3 PUFA and E₂ decreased the bone expressions of IL-7, TNF-α and PPAR-γ, and increased the bone expression of oestrogen receptor-α. n-3 PUFA in the presence of E₂ and E₂ alone significantly decreased the bone expressions of IL-1β and IL-6 and increased the bone expression of RUNX2. E₂ significantly decreased the serum levels of bone turnover markers and the bone expression of receptor activator of NF-κB ligand, but decreased the bone expression of osteoprotegerin. The combined use of n-3 PUFA and E₂ exerted synergistic bone-protective efficacy through up-regulation of RUNX2, an essential transcription factor for bone formation, as well as the suppression of bone-resorbing cytokine IL-1β.
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