Anti-inflammatory effects of Nelumbo leaf extracts and identification of their metabolitesopen access
- Authors
- Park, Eunkyo; Kim, Gyoung Deuck; Go, Min-Sun; Kwon, Dodan; Jung, In-Kyung; Auh, Joong Hyuck; Kim, Jung-Hyun
- Issue Date
- Aug-2017
- Publisher
- KOREAN NUTRITION SOC
- Keywords
- Nelumbo leaf; inflammation; metabolomics; macrophage
- Citation
- NUTRITION RESEARCH AND PRACTICE, v.11, no.4, pp 265 - 274
- Pages
- 10
- Journal Title
- NUTRITION RESEARCH AND PRACTICE
- Volume
- 11
- Number
- 4
- Start Page
- 265
- End Page
- 274
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/4161
- DOI
- 10.4162/nrp.2017.11.4.265
- ISSN
- 1976-1457
2005-6168
- Abstract
- BACKGROUND/OBJECTIVES: Nelumbo leaves have been used in traditional medicine to treat bleeding, gastritis, hemorrhoids, and halitosis. However, their mechanisms have not been elucidated. MATERIALS/METHODS: The present study prepared two Nelumbo leaf extracts (NLEs) using water or 50% ethanol. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha (TNF-alpha), interleukin (1L)-1 beta, and IL-6 and nitric oxide (NO) and prostaglandin E-2 (PGE(2)) productions were assessed. To determine the anti-inflammatory mechanism of NLEs, we measured nuclear factor-kappa B (NF-kappa B) activity. Major metabolites of NLEs were also analyzed and quantified. RESULTS: NLEs effectively reduced the expression and productions of pro-inflammatory mediators such as IL-1 beta, IL-6, TNF-alpha, PGE(2), and NO. NLEs also reduced NF-kappa B activity by inhibiting inhibitor of NF-kappa B phosphorylation. Both extracts contained catechin and quercetin, bioactive compounds of NLEs. CONCLUSIONS: In this study, we showed that NLEs could be used to inhibit NF-kappa B-mediated inflammatory responses. In addition, our data support the idea that NLEs can ameliorate disease conditions involving chronic inflammation.
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